Correlation between the Hypoosmotic SwellingT and DNA Fragmentation Assessed by the TUNEL Assay in Asthenozoospermia
نویسندگان
چکیده
منابع مشابه
Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay.
BACKGROUND In this study we present the physiological changes observed in ejaculated spermatozoa of normospermic men after exposure to hydrogen peroxide (H(2)O(2)) or gamma irradiation. METHODS Motility changes as well as membrane and DNA-damage were determined in spermatozoa after incubation with 25 micromol/l of H(2)O(2) during increasing intervals of time (0--60 min and after 24 h) or afte...
متن کاملHuman sperm DNA fragmentation: correlation of TUNEL results as assessed by flow cytometry and optical microscopy.
An association between DNA fragmentation in sperm determined by the terminal deoxynucleotidyl transferase [TdT]-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) assay and the incidence of reproductive failure has been reported, either using flow cytometry or optical microscopy. However, the results obtained using each of these two approaches are different. Since there is a re...
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ژورنال
عنوان ژورنال: Siriraj Medical Journal
سال: 2019
ISSN: 2629-995X,2228-8082
DOI: 10.33192/smj.2019.02